Isolation and full-genome sequences of Japanese encephalitis virus genotype I strains from Cambodian human patients, mosquitoes and pigs.
Identifieur interne : 000917 ( Main/Exploration ); précédent : 000916; suivant : 000918Isolation and full-genome sequences of Japanese encephalitis virus genotype I strains from Cambodian human patients, mosquitoes and pigs.
Auteurs : Veasna Duong [Cambodge] ; Rithy Choeung [Cambodge] ; Christopher Gorman [Cambodge] ; Denis Laurent [Cambodge] ; Yoann Crabol [Cambodge] ; Channa Mey [Cambodge] ; Borin Peng [Cambodge] ; Juliette Di Francesco [Cambodge] ; Vibol Hul [Cambodge] ; Heng Sothy [Cambodge] ; Ky Santy [Cambodge] ; Beat Richner [Cambodge] ; Jean-David Pommier [Cambodge] ; San Sorn [Cambodge] ; Véronique Chevalier [France] ; Philippe Buchy [Singapour] ; Xavier De Lamballerie [France] ; Julien Cappelle [France] ; Paul Francis Horwood [Australie] ; Philippe Dussart [Cambodge]Source :
- The Journal of general virology [ 1465-2099 ] ; 2017.
Descripteurs français
- KwdFr :
- Animaux, Cambodge, Culicidae (virologie), Encéphalite japonaise (médecine vétérinaire), Encéphalite japonaise (virologie), Enfant, Enfant d'âge préscolaire, Femelle, Génome viral, Génotype, Humains, Maladies des porcs (virologie), Mâle, Nourrisson, Phylogénie, Suidae, Virus de l'encéphalite japonaise (espèce) (), Virus de l'encéphalite japonaise (espèce) (génétique), Virus de l'encéphalite japonaise (espèce) (isolement et purification), Études de cohortes.
- MESH :
- génétique : Virus de l'encéphalite japonaise (espèce).
- isolement et purification : Virus de l'encéphalite japonaise (espèce).
- médecine vétérinaire : Encéphalite japonaise.
- virologie : Culicidae, Encéphalite japonaise, Maladies des porcs.
- Animaux, Cambodge, Enfant, Enfant d'âge préscolaire, Femelle, Génome viral, Génotype, Humains, Mâle, Nourrisson, Phylogénie, Suidae, Virus de l'encéphalite japonaise (espèce), Études de cohortes.
English descriptors
- KwdEn :
- Animals, Cambodia, Child, Child, Preschool, Cohort Studies, Culicidae (virology), Encephalitis Virus, Japanese (classification), Encephalitis Virus, Japanese (genetics), Encephalitis Virus, Japanese (isolation & purification), Encephalitis, Japanese (veterinary), Encephalitis, Japanese (virology), Female, Genome, Viral, Genotype, Humans, Infant, Male, Phylogeny, Swine, Swine Diseases (virology).
- MESH :
- classification : Encephalitis Virus, Japanese.
- genetics : Encephalitis Virus, Japanese.
- isolation & purification : Encephalitis Virus, Japanese.
- veterinary : Encephalitis, Japanese.
- virology : Culicidae, Encephalitis, Japanese, Swine Diseases.
- Animals, Cambodia, Child, Child, Preschool, Cohort Studies, Female, Genome, Viral, Genotype, Humans, Infant, Male, Phylogeny, Swine.
Abstract
Japanese encephalitis remains the most important cause of viral encephalitis in humans in several southeast Asian countries, including Cambodia, causing at least 65 000 cases of encephalitis per year. This vector-borne viral zoonosis - caused by Japanese encephalitis virus (JEV) - is considered to be a rural disease and is transmitted by mosquitoes, with birds and pigs being the natural reservoirs, while humans are accidental hosts. In this study we report the first two JEV isolations in Cambodia from human encephalitis cases from two studies on the aetiology of central nervous system disease, conducted at the two major paediatric hospitals in the country. We also report JEV isolation from Culextritaeniorhynchus mosquitoes and from pig samples collected in two farms, located in peri-urban and rural areas. Out of 11 reverse-transcription polymerase chain reaction-positive original samples, we generated full-genome sequences from 5 JEV isolates. Five additional partial sequences of the JEV NS3 gene from viruses detected in five pigs and one complete coding sequence of the envelope gene of a strain identified in a pig were generated. Phylogenetic analyses revealed that JEV detected in Cambodia belonged to genotype I and clustered in two clades: genotype I-a, mainly comprising strains from Thailand, and genotype I-b, comprising strains from Vietnam that dispersed northwards to China. Finally, in this study, we provide proof that the sequenced JEV strains circulate between pigs, Culex tritaeniorhynchus and humans in the Phnom Penh vicinity.
DOI: 10.1099/jgv.0.000892
PubMed: 28840803
Affiliations:
- Australie, Cambodge, France, Singapour
- Auvergne-Rhône-Alpes, Languedoc-Roussillon, Occitanie (région administrative), Provence-Alpes-Côte d'Azur, Rhône-Alpes
- Marcy-l'étoile, Marseille, Montpellier
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000620
- to stream PubMed, to step Curation: 000618
- to stream PubMed, to step Checkpoint: 000618
- to stream Ncbi, to step Merge: 004D39
- to stream Ncbi, to step Curation: 004D39
- to stream Ncbi, to step Checkpoint: 004D39
- to stream Main, to step Merge: 000912
- to stream Main, to step Curation: 000917
Le document en format XML
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Penh</wicri:noRegion></affiliation></author><author><name sortKey="Di Francesco, Juliette" sort="Di Francesco, Juliette" uniqKey="Di Francesco J" first="Juliette" last="Di Francesco">Juliette Di Francesco</name><affiliation wicri:level="1"><nlm:affiliation>3Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>3Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author><author><name sortKey="Hul, Vibol" sort="Hul, Vibol" uniqKey="Hul V" first="Vibol" last="Hul">Vibol Hul</name><affiliation wicri:level="1"><nlm:affiliation>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia.</nlm:affiliation><country 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Penh</wicri:noRegion></affiliation></author><author><name sortKey="Richner, Beat" sort="Richner, Beat" uniqKey="Richner B" first="Beat" last="Richner">Beat Richner</name><affiliation wicri:level="1"><nlm:affiliation>2Kantha Bopha Hospital, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>2Kantha Bopha Hospital, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author><author><name sortKey="Pommier, Jean David" sort="Pommier, Jean David" uniqKey="Pommier J" first="Jean-David" last="Pommier">Jean-David Pommier</name><affiliation wicri:level="1"><nlm:affiliation>3Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>3Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author><author><name sortKey="Sorn, San" sort="Sorn, San" uniqKey="Sorn S" first="San" last="Sorn">San Sorn</name><affiliation wicri:level="1"><nlm:affiliation>4General Directorate of Animal Health and Production, Ministry of Agriculture, Forestry and Fisheries, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>4General Directorate of Animal Health and Production, Ministry of Agriculture, Forestry and Fisheries, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author><author><name sortKey="Chevalier, Veronique" sort="Chevalier, Veronique" uniqKey="Chevalier V" first="Véronique" last="Chevalier">Véronique Chevalier</name><affiliation wicri:level="3"><nlm:affiliation>3Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia 5UMR ASTRE, 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139234</wicri:regionArea><wicri:noRegion>139234</wicri:noRegion></affiliation></author><author><name sortKey="De Lamballerie, Xavier" sort="De Lamballerie, Xavier" uniqKey="De Lamballerie X" first="Xavier" last="De Lamballerie">Xavier De Lamballerie</name><affiliation wicri:level="3"><nlm:affiliation>7UMR D 190 'Emergence des Pathologies Virales' (Aix-Marseille UnivIRD French Institute of Research for Development, EHESP French School of Public Health), Marseille, France 8IHU Méditerranée Infection, APHM Public Hospitals of Marseille, 13005 Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>7UMR D 190 'Emergence des Pathologies Virales' (Aix-Marseille UnivIRD French Institute of Research for Development, EHESP French School of Public Health), Marseille, France 8IHU Méditerranée Infection, APHM Public Hospitals of Marseille, 13005 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Cappelle, Julien" sort="Cappelle, Julien" uniqKey="Cappelle J" first="Julien" last="Cappelle">Julien Cappelle</name><affiliation wicri:level="3"><nlm:affiliation>3Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia 5UMR ASTRE, CIRAD-BIOS, F-34398, Montpellier, France 9UMR EPIA, INRA, VetAgro Sup, Univ Lyon, F-69280, Marcy-l'étoile, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>3Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia 5UMR ASTRE, CIRAD-BIOS, F-34398, Montpellier, France 9UMR EPIA, INRA, VetAgro Sup, Univ Lyon, F-69280, Marcy-l'étoile</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Marcy-l'étoile</settlement></placeName></affiliation></author><author><name sortKey="Horwood, Paul Francis" sort="Horwood, Paul Francis" uniqKey="Horwood P" first="Paul Francis" last="Horwood">Paul Francis Horwood</name><affiliation wicri:level="1"><nlm:affiliation>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia 10Australian Institute of Tropical Health and Medicine, James Cook University, PO Box 6811, Cairns, QLD 4870, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia 10Australian Institute of Tropical Health and Medicine, James Cook University, PO Box 6811, Cairns, QLD 4870</wicri:regionArea><wicri:noRegion>QLD 4870</wicri:noRegion></affiliation></author><author><name sortKey="Dussart, Philippe" sort="Dussart, Philippe" uniqKey="Dussart P" first="Philippe" last="Dussart">Philippe Dussart</name><affiliation wicri:level="1"><nlm:affiliation>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28840803</idno><idno type="pmid">28840803</idno><idno type="doi">10.1099/jgv.0.000892</idno><idno type="wicri:Area/PubMed/Corpus">000620</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000620</idno><idno type="wicri:Area/PubMed/Curation">000618</idno><idno type="wicri:explorRef" 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Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author><author><name sortKey="Choeung, Rithy" sort="Choeung, Rithy" uniqKey="Choeung R" first="Rithy" last="Choeung">Rithy Choeung</name><affiliation wicri:level="1"><nlm:affiliation>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author><author><name sortKey="Gorman, Christopher" sort="Gorman, Christopher" uniqKey="Gorman C" first="Christopher" last="Gorman">Christopher Gorman</name><affiliation wicri:level="1"><nlm:affiliation>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author><author><name sortKey="Laurent, Denis" sort="Laurent, Denis" uniqKey="Laurent D" first="Denis" last="Laurent">Denis Laurent</name><affiliation wicri:level="1"><nlm:affiliation>2Kantha Bopha Hospital, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>2Kantha Bopha Hospital, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author><author><name sortKey="Crabol, Yoann" sort="Crabol, Yoann" uniqKey="Crabol Y" first="Yoann" last="Crabol">Yoann Crabol</name><affiliation wicri:level="1"><nlm:affiliation>3Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>3Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author><author><name sortKey="Mey, Channa" sort="Mey, Channa" uniqKey="Mey C" first="Channa" last="Mey">Channa Mey</name><affiliation wicri:level="1"><nlm:affiliation>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author><author><name sortKey="Peng, Borin" sort="Peng, Borin" uniqKey="Peng B" first="Borin" last="Peng">Borin Peng</name><affiliation wicri:level="1"><nlm:affiliation>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author><author><name sortKey="Di Francesco, Juliette" sort="Di Francesco, Juliette" uniqKey="Di Francesco J" first="Juliette" last="Di Francesco">Juliette Di Francesco</name><affiliation wicri:level="1"><nlm:affiliation>3Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>3Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author><author><name sortKey="Hul, Vibol" sort="Hul, Vibol" uniqKey="Hul V" first="Vibol" last="Hul">Vibol Hul</name><affiliation wicri:level="1"><nlm:affiliation>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author><author><name sortKey="Sothy, Heng" sort="Sothy, Heng" uniqKey="Sothy H" first="Heng" last="Sothy">Heng Sothy</name><affiliation wicri:level="1"><nlm:affiliation>2Kantha Bopha Hospital, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>2Kantha Bopha Hospital, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author><author><name sortKey="Santy, Ky" sort="Santy, Ky" uniqKey="Santy K" first="Ky" last="Santy">Ky Santy</name><affiliation wicri:level="1"><nlm:affiliation>2Kantha Bopha Hospital, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>2Kantha Bopha Hospital, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author><author><name sortKey="Richner, Beat" sort="Richner, Beat" uniqKey="Richner B" first="Beat" last="Richner">Beat Richner</name><affiliation wicri:level="1"><nlm:affiliation>2Kantha Bopha Hospital, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>2Kantha Bopha Hospital, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author><author><name sortKey="Pommier, Jean David" sort="Pommier, Jean David" uniqKey="Pommier J" first="Jean-David" last="Pommier">Jean-David Pommier</name><affiliation wicri:level="1"><nlm:affiliation>3Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>3Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author><author><name sortKey="Sorn, San" sort="Sorn, San" uniqKey="Sorn S" first="San" last="Sorn">San Sorn</name><affiliation wicri:level="1"><nlm:affiliation>4General Directorate of Animal Health and Production, Ministry of Agriculture, Forestry and Fisheries, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>4General Directorate of Animal Health and Production, Ministry of Agriculture, Forestry and Fisheries, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author><author><name sortKey="Chevalier, Veronique" sort="Chevalier, Veronique" uniqKey="Chevalier V" first="Véronique" last="Chevalier">Véronique Chevalier</name><affiliation wicri:level="3"><nlm:affiliation>3Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia 5UMR ASTRE, CIRAD-BIOS, F-34398, Montpellier, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>3Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia 5UMR ASTRE, CIRAD-BIOS, F-34398, Montpellier</wicri:regionArea><placeName><settlement type="city">Montpellier</settlement><region type="region" nuts="2">Occitanie (région administrative)</region><region type="old region" nuts="2">Languedoc-Roussillon</region></placeName></affiliation></author><author><name sortKey="Buchy, Philippe" sort="Buchy, Philippe" uniqKey="Buchy P" first="Philippe" last="Buchy">Philippe Buchy</name><affiliation wicri:level="1"><nlm:affiliation>6GlaxoSmithKline Vaccines R&D, 23 Rochester Park, 139234, Singapore.</nlm:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>6GlaxoSmithKline Vaccines R&D, 23 Rochester Park, 139234</wicri:regionArea><wicri:noRegion>139234</wicri:noRegion></affiliation></author><author><name sortKey="De Lamballerie, Xavier" sort="De Lamballerie, Xavier" uniqKey="De Lamballerie X" first="Xavier" last="De Lamballerie">Xavier De Lamballerie</name><affiliation wicri:level="3"><nlm:affiliation>7UMR D 190 'Emergence des Pathologies Virales' (Aix-Marseille UnivIRD French Institute of Research for Development, EHESP French School of Public Health), Marseille, France 8IHU Méditerranée Infection, APHM Public Hospitals of Marseille, 13005 Marseille, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>7UMR D 190 'Emergence des Pathologies Virales' (Aix-Marseille UnivIRD French Institute of Research for Development, EHESP French School of Public Health), Marseille, France 8IHU Méditerranée Infection, APHM Public Hospitals of Marseille, 13005 Marseille</wicri:regionArea><placeName><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region><settlement type="city">Marseille</settlement></placeName></affiliation></author><author><name sortKey="Cappelle, Julien" sort="Cappelle, Julien" uniqKey="Cappelle J" first="Julien" last="Cappelle">Julien Cappelle</name><affiliation wicri:level="3"><nlm:affiliation>3Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia 5UMR ASTRE, CIRAD-BIOS, F-34398, Montpellier, France 9UMR EPIA, INRA, VetAgro Sup, Univ Lyon, F-69280, Marcy-l'étoile, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>3Epidemiology and Public Health Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia 5UMR ASTRE, CIRAD-BIOS, F-34398, Montpellier, France 9UMR EPIA, INRA, VetAgro Sup, Univ Lyon, F-69280, Marcy-l'étoile</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region><settlement type="city">Marcy-l'étoile</settlement></placeName></affiliation></author><author><name sortKey="Horwood, Paul Francis" sort="Horwood, Paul Francis" uniqKey="Horwood P" first="Paul Francis" last="Horwood">Paul Francis Horwood</name><affiliation wicri:level="1"><nlm:affiliation>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia 10Australian Institute of Tropical Health and Medicine, James Cook University, PO Box 6811, Cairns, QLD 4870, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia 10Australian Institute of Tropical Health and Medicine, James Cook University, PO Box 6811, Cairns, QLD 4870</wicri:regionArea><wicri:noRegion>QLD 4870</wicri:noRegion></affiliation></author><author><name sortKey="Dussart, Philippe" sort="Dussart, Philippe" uniqKey="Dussart P" first="Philippe" last="Dussart">Philippe Dussart</name><affiliation wicri:level="1"><nlm:affiliation>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh, Cambodia.</nlm:affiliation><country xml:lang="fr">Cambodge</country><wicri:regionArea>1Virology Unit, Institut Pasteur du Cambodge, Institut Pasteur International Network, PO Box 983, Phnom Penh</wicri:regionArea><wicri:noRegion>Phnom Penh</wicri:noRegion></affiliation></author></analytic><series><title level="j">The Journal of general virology</title><idno type="eISSN">1465-2099</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cambodia</term><term>Child</term><term>Child, Preschool</term><term>Cohort Studies</term><term>Culicidae (virology)</term><term>Encephalitis Virus, Japanese (classification)</term><term>Encephalitis Virus, Japanese (genetics)</term><term>Encephalitis Virus, Japanese (isolation & purification)</term><term>Encephalitis, Japanese (veterinary)</term><term>Encephalitis, Japanese (virology)</term><term>Female</term><term>Genome, Viral</term><term>Genotype</term><term>Humans</term><term>Infant</term><term>Male</term><term>Phylogeny</term><term>Swine</term><term>Swine Diseases (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cambodge</term><term>Culicidae (virologie)</term><term>Encéphalite japonaise (médecine vétérinaire)</term><term>Encéphalite japonaise (virologie)</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Génome viral</term><term>Génotype</term><term>Humains</term><term>Maladies des porcs (virologie)</term><term>Mâle</term><term>Nourrisson</term><term>Phylogénie</term><term>Suidae</term><term>Virus de l'encéphalite japonaise (espèce) ()</term><term>Virus de l'encéphalite japonaise (espèce) (génétique)</term><term>Virus de l'encéphalite japonaise (espèce) (isolement et purification)</term><term>Études de cohortes</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Encephalitis Virus, Japanese</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Encephalitis Virus, Japanese</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus de l'encéphalite japonaise (espèce)</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Encephalitis Virus, Japanese</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus de l'encéphalite japonaise (espèce)</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Encéphalite japonaise</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Encephalitis, Japanese</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Culicidae</term><term>Encéphalite japonaise</term><term>Maladies des porcs</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Culicidae</term><term>Encephalitis, Japanese</term><term>Swine Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cambodia</term><term>Child</term><term>Child, Preschool</term><term>Cohort Studies</term><term>Female</term><term>Genome, Viral</term><term>Genotype</term><term>Humans</term><term>Infant</term><term>Male</term><term>Phylogeny</term><term>Swine</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cambodge</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Génome viral</term><term>Génotype</term><term>Humains</term><term>Mâle</term><term>Nourrisson</term><term>Phylogénie</term><term>Suidae</term><term>Virus de l'encéphalite japonaise (espèce)</term><term>Études de cohortes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Japanese encephalitis remains the most important cause of viral encephalitis in humans in several southeast Asian countries, including Cambodia, causing at least 65 000 cases of encephalitis per year. This vector-borne viral zoonosis - caused by Japanese encephalitis virus (JEV) - is considered to be a rural disease and is transmitted by mosquitoes, with birds and pigs being the natural reservoirs, while humans are accidental hosts. In this study we report the first two JEV isolations in Cambodia from human encephalitis cases from two studies on the aetiology of central nervous system disease, conducted at the two major paediatric hospitals in the country. We also report JEV isolation from Culextritaeniorhynchus mosquitoes and from pig samples collected in two farms, located in peri-urban and rural areas. Out of 11 reverse-transcription polymerase chain reaction-positive original samples, we generated full-genome sequences from 5 JEV isolates. Five additional partial sequences of the JEV NS3 gene from viruses detected in five pigs and one complete coding sequence of the envelope gene of a strain identified in a pig were generated. Phylogenetic analyses revealed that JEV detected in Cambodia belonged to genotype I and clustered in two clades: genotype I-a, mainly comprising strains from Thailand, and genotype I-b, comprising strains from Vietnam that dispersed northwards to China. Finally, in this study, we provide proof that the sequenced JEV strains circulate between pigs, Culex tritaeniorhynchus and humans in the Phnom Penh vicinity.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Cambodge</li><li>France</li><li>Singapour</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Languedoc-Roussillon</li><li>Occitanie (région administrative)</li><li>Provence-Alpes-Côte d'Azur</li><li>Rhône-Alpes</li></region><settlement><li>Marcy-l'étoile</li><li>Marseille</li><li>Montpellier</li></settlement></list><tree><country name="Cambodge"><noRegion><name sortKey="Duong, Veasna" sort="Duong, Veasna" uniqKey="Duong V" first="Veasna" last="Duong">Veasna Duong</name></noRegion><name sortKey="Choeung, Rithy" sort="Choeung, Rithy" uniqKey="Choeung R" first="Rithy" last="Choeung">Rithy Choeung</name><name sortKey="Crabol, Yoann" sort="Crabol, Yoann" uniqKey="Crabol Y" first="Yoann" last="Crabol">Yoann Crabol</name><name sortKey="Di Francesco, Juliette" sort="Di Francesco, Juliette" uniqKey="Di Francesco J" first="Juliette" last="Di Francesco">Juliette Di Francesco</name><name sortKey="Dussart, Philippe" sort="Dussart, Philippe" uniqKey="Dussart P" first="Philippe" last="Dussart">Philippe Dussart</name><name sortKey="Gorman, Christopher" sort="Gorman, Christopher" uniqKey="Gorman C" first="Christopher" last="Gorman">Christopher Gorman</name><name sortKey="Hul, Vibol" sort="Hul, Vibol" uniqKey="Hul V" first="Vibol" last="Hul">Vibol Hul</name><name sortKey="Laurent, Denis" sort="Laurent, Denis" uniqKey="Laurent D" first="Denis" last="Laurent">Denis Laurent</name><name sortKey="Mey, Channa" sort="Mey, Channa" uniqKey="Mey C" first="Channa" last="Mey">Channa Mey</name><name sortKey="Peng, Borin" sort="Peng, Borin" uniqKey="Peng B" first="Borin" last="Peng">Borin Peng</name><name sortKey="Pommier, Jean David" sort="Pommier, Jean David" uniqKey="Pommier J" first="Jean-David" last="Pommier">Jean-David Pommier</name><name sortKey="Richner, Beat" sort="Richner, Beat" uniqKey="Richner B" first="Beat" last="Richner">Beat Richner</name><name sortKey="Santy, Ky" sort="Santy, Ky" uniqKey="Santy K" first="Ky" last="Santy">Ky Santy</name><name sortKey="Sorn, San" sort="Sorn, San" uniqKey="Sorn S" first="San" last="Sorn">San Sorn</name><name sortKey="Sothy, Heng" sort="Sothy, Heng" uniqKey="Sothy H" first="Heng" last="Sothy">Heng Sothy</name></country><country name="France"><region name="Occitanie (région administrative)"><name sortKey="Chevalier, Veronique" sort="Chevalier, Veronique" uniqKey="Chevalier V" first="Véronique" last="Chevalier">Véronique Chevalier</name></region><name sortKey="Cappelle, Julien" sort="Cappelle, Julien" uniqKey="Cappelle J" first="Julien" last="Cappelle">Julien Cappelle</name><name sortKey="De Lamballerie, Xavier" sort="De Lamballerie, Xavier" uniqKey="De Lamballerie X" first="Xavier" last="De Lamballerie">Xavier De Lamballerie</name></country><country name="Singapour"><noRegion><name sortKey="Buchy, Philippe" sort="Buchy, Philippe" uniqKey="Buchy P" first="Philippe" last="Buchy">Philippe Buchy</name></noRegion></country><country name="Australie"><noRegion><name sortKey="Horwood, Paul Francis" sort="Horwood, Paul Francis" uniqKey="Horwood P" first="Paul Francis" last="Horwood">Paul Francis Horwood</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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